Prof. Gazit - Skeletal Biotech Lab

International Presentations: (Last two years)

56th Annual Meeting of the Orthopaedic Research Society, 2010, New Orleans, LA:

Workshop 6: Imaging Stem Cell Fate and Function
Organizers: Dan Gazit, PhD, DMD, and Edward M Schwarz, PhD.

Stem cells hold tremendous potential for the development of new therapeutic strategies for tissue regeneration. However much has to be learned of the fate and function of these cells in the living tissue. Advanced molecular and micro imaging methods play an increasingly important role in the rigorous evaluation of stem cell-based tissue regenerative strategies. Imaging techniques such as high-resolution micro-Computed Tomography0 (micro-CT), Single Photon Emission Computed Tomography (SPECT), Positron Emission Tomography (PET), Bioluminescence and Fluorescence imaging provide non-invasive, real-time, quantitative tools for cell and tissue analysis. We are now able to track implanted cells, monitor their differentiation and observe tissue development in vivo. In this workshop, we will review the aforementioned imaging techniques and their application in stem cells- based skeletal tissue regeneration.

High Performance Nuclear Imaging in Regenerative Medicine
Douglas J Wagenaar, Northridge, CA

Imaging as a Window into Stem Cell Biology
Christopher H. Contag, Stanford, CA

Functional Imaging for Skeletal Stem Cell Based Tissue engineering
Dan Gazit, PhD, DMD, Los Angeles, CA

IV International Symposium - Advanced Therapy & Stem Cells, Recife, Brazil:

Mesenchymal Stem Cells: Immunoisolation and Clinical Application in Skeletal Regeneration.
Zulma Gazit.

The Role of Adult Stem Cells in Degeneration and Regeneration of the Intervertebral Disc.
Zulma Gazit.

Functional Imaging for Skeletal Stem Cell Based Tissue engineering.
Dan Gazit.

Stem cell based Skeletal Tissue Engineering.
Dan Gazit.

World Molecular Imaging Congress 2009, Montreal, Canada:

Bioluminescence, Transgenic Reporter Mice, Fibered Confocal Microscopy and Micro CT Imaging Demonstrate the Effect of Synthetic Oxygen Carriers on Stem Cell-Based Bone Regeneration.
Gadi Pelled, Nadav Kimelman-Bleich, Yoram Zilberman, Wafa Tawackoli, Zulma Gazit, Dan Gazit.

In Vivo and Ex Vivo Micro MRI Analysis of Native and Stem Cell-Based Engineered Annulus Fibrosus.
Gadi Pelled, Yoram Zilberman, Galit Saar, Nadav Kimelman-Bleich, Hadassah Shinar, Keren Keinan-Adamsky, David Kaplan, Carmen Preda, Dan Gazit, Gil Navon.

Bioluminescence and Micro CT imaging for Quantifying Bone Tissue Regeneration using rAAV-BMP2-Coated Calvarial Allografts.
Gadi Pelled, Ayelet Ben Arav, Wafa Tawackoli, Zulma Gazit, Edward M. Schwarz, Dan Gazit.

In Vivo Monitoring of Electroporation - Based Non-Union Fracture Repair using Bioluminescence and Micro CT Imaging.
Gadi Pelled, Nadav Kimelman-Bleich, Olga Mizrahi, Yoram Zilberman, Dan Gazit.

12th Annual Meeting of the American Society of Gene & Cell Therapy, 2009, San Diego, CA:

Craniofacial Bone Tissue Regeneration using rAAV-BMP2-Coated Calvarial Allografts.
Zulma Gazit, Ayelet Ben Arav, Coleen Hock, David G Reynolds, Gadi Pelled, Yoram Zilberman, Edward M. Schwarz, Gazit D.

In Vivo Tenocytic Differentiation of Primary Mesenchymal Stem Cells by Gene Delivery of Smad8.
Zulma Gazit, Yair Steinhardt, Ayelet Ben Arav, Yoram Zilberman, Andrea Hoffmann, Gerhard Gross, Gadi Pelled, Dan Gazit.

Smad8/BMP-2 Engineered Mesenchymal Stem Cells Induce Rapid Achilles Tendon Full Defect Regeneration.
Ayelet Ben Arav, Jess G Snedeker, Yoram Zilberman, Zulma Gazit, Ralph Müller, Gadi Pelled, Dan Gazit.

In Vivo Electroporation of BMP-9 Gene Induces Bone Regeneration and Non-Union Fracture Repair.
Gadi Pelled, Nadav Kimelman- Bleich, Yoram Zilberman, J Li, Gregory A Helm, Dan Gazit.

Synthetic Oxygen Carriers Enhance Stem Cell-Based Spinal Fusion and Bone Defect Regeneration.
Zulma Gazit, Nadav Kimelman- Bleich, Gadi Pelled, Yoram Zilberman, Carmen Prada, David L Kaplan, Dan Gazit.

55th Annual Meeting of the Orthopaedic Research Society, 2009, Las Vegas, NV:

Injectable Engineered Stem Cells Induce Mechanically Rigid Posterior Spine Fusion.
Dan Gazit, Dima Sheyn, Martin Rüthemann, Gadi Pelled, Yoram Zilberman, Jess G Snedeker.

Advanced MR-Analysis of Native and Stem Cell-Based Engineered Annulus Fibrosus.
Dan Gazit, Yoram Zilberman, Nadav Kimelman, Gadi Pelled, Galit Saar, Hadassah Shinar, Keren Keinan-Adamsky, David Kaplan, Carmen Preda, Gil Navon.

Synthetic Oxygen Carriers Enhance Bone Defect Regeneration Induced by BMP-Expressing Mesenchymal Stem Cells.
Gadi Pelled, Nadav Kimelman, Yoram Zilberman, Zulma Gazit, Dan Gazit.

In Vivo Bone Tissue Regeneration Using rAAV-BMP2-Coated Allografts.
Dan Gazit, Ayelet Ben-Arav, Gadi Pelled, Yoram Zilberman, Colleen Hock, David Reinholds, Edward Schwarz.

Non Union Bone Defect Regeneration using In Vivo Electroporation-Based Gene Delivery.
Gadi Pelled, Nadav Kimelman, Yoram Zilberman, Gregory Helm, J Li, Dan Gazit.

The TERMIS North America 2008 Annual Conference, San Diego, CA:

Injectable Engineered Stem Cells Induce Mechanically Rigid Posterior Spine Fusion.
Dan Gazit, Dima Sheyn, Martin Rüthemann, Gadi Pelled, Yoram Zilberman, Jess G Snedeker.

In vivo tracking of quantum-dot labeled human mesenchymal stem cells in SCID mice.
Bhranti S Shah, Dima Sheyn, Gadi Pelled, RY Yang, Dan Gazit, Jeremy J Mao.

Synthetic Oxygen Carriers Enhances Engineered MSC-Based In Vivo Bone Formation.
Nadav Kimelmann, Gadi Pelled, Yoram Zilberman, Zulma Gazit, Dan Gazit.

Micro Imaging System for Analyzing The Biomechanics of Engineered Tendons In vivo.
Gadi Pelled, Jess G Snedeker, Ayelet Ben Arav, Yoram Zilberman, Dan Gazit.

Quantitative Magnetic Resonance Imaging of Native and Stem Cell-Based Tissue-Engineered Annulus Fibrosus.
Pelled G, Zilberman Y, Kimelman-Bleich N, Saar G, Shinar H, Keinan-Adamsky K, Kaplan DL, Preda C, Navon G, Gazit D.

Craniofacial Bone Tissue Engineering using rAAV-BMP2-Coated Calvaria Allografts.
Dan Gazit, Ayelet Ben Arav, Gadi Pelledm Yoram Zilberman, Coleen hock, David g Reynolds, Edward M Schwarz.

Microstructural Finite Element Model and Computed Tomography Analysis of Non Union Fracture Repaired By Engineered Mesenchymal Stem Cells.
Gadi pelled, Harry van Lenthe, Ilan Kallai, Yoram Zilberman, Ralph Müller, Dan Gazit.

16th annual scientific meeting of the International Spine Intervention Society 2008, Las Vegas, NV:

Injectable Adult Stem Cells as a Novel Therapeutic Platform for Anterior and Posterior Spinal Fusion.
Dima Sheyn, Gadi Pelled, Zulma Gazit, Christopher J. Zarembinski MD, Neel Anand MD, Patrick J. Johnson MD, and Dan Gazit.

(Awarded a prize for Best Science Paper)

Israeli Stem Cells Society, 2nd International stem cells meeting 2008, Tel-Aviv, Israel:

Adipose tissue engineering mediated by genetically engineered MSCs.
Dima Sheyn, Gadi Pelled, Yoram Zilberman, Dan Gazit and Zulma Gazit.

Adult Stem Cell-based Bone Formation Enhanced by Synthetic Oxygen Carrier.
Nadav Kimelman-Bleich, Yoram Zilberman, Gadi Pelled, David L. Kaplan, Zulma Gazit, Dan Gazit.

Hilton Head Workshop of Regenerative Medicine, 2008:

Micro CT-based Biomechanical Analysis of Nonunion Fracture Repaired by Genetically Modified Mesenchymal Stem Cells.
Dima Sheyn, Gadi Pelled, Harry van Lenthe, Ilan Kallai, Yoram Zilberman, Ralph Müller, Dan Gazit.

54th Annual Meeting of the Orthopaedic Research Society, 2008, San Francisco, CA:

Assessing the Stiffness of Lumbar Spinal Fusion in a Murine Model.
Rüthemann Martin, Sheyn Dima, Zilberman Yoram, Pelled Gadi, Gazit Dan; Snedeker Jess G.

Multimodal Imaging of Whole Joints Allows Quantification of Cartilage and Bone Degeneration in a Murine Model of Osteoarthritis.
Kathryn S Stok, Gadi Pelled, Yoram Zilberman, Ilan Kallai, Dan Gazit, Ralph Müller.

An Analytical Model for Elucidating Tendon Tissue Structure and Biomechanical Function from In Vivo Cellular Confocal Microscopy Images.
Snedeker G Jess, Gadi Pelled, Yoram Zilberman, Ayelet Ben Arav, Ralph Müller, Dan Gazit.

Quantitative, Structural and Image-based Mechanical Analysis of Nonunion Fracture Repaired by Genetically Engineered Mesenchymal Stem Cells.
Gadi Pelled, Harry van Lenthe, Ilan Kallai, Yoram Zilberman, Ralph Müller, Dan Gazit.

Silk Gel Enriched with Synthetic Oxygen Carriers Enhances Engineered MSC-Based In Vivo Bone Formation.
Nadav Kimelman, Gadi Pelled, Carmen Preda, Yoram Zilberman, Heinz Redl, David Kaplan, Dan Gazit.