@article {17117,
	title = {Ovariectomy and genes encoding core circadian regulatory proteins in murine bone},
	journal = {Osteoporos Int},
	volume = {22},
	number = {5},
	year = {2011},
	note = {Smith, B J Sutton, G M Wu, X Yu, G Goh, B C Hebert, T Pelled, G Gazit, Z Gazit, D Butler, A A Gimble, J M eng MYNCBI/CB/NCI NIH HHS/ P30 DK072476/DK/NIDDK NIH HHS/ 1P30 DK072476/DK/NIDDK NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov{\textquoteright}t England 2010/07/02 06:00 Osteoporos Int. 2011 May;22(5):1633-9. doi: 10.1007/s00198-010-1325-z. Epub 2010 Jul 1.},
	pages = {1633-9},
	type = {Journal Article},
	abstract = {SUMMARY: This study investigated the influence of ovarian hormone deficiency on core circadian regulatory protein (CCRP) in the context of bone loss. Our data suggest that ovarian hormone deficiency disrupts diurnal rhythmicity and CCRP expression in bone. Further studies should determine if chronobiology provides a novel therapeutic target for osteoporosis intervention. INTRODUCTION: CCRP synchronize metabolic activities and display an oscillatory expression profile in murine bone. In vitro studies using bone marrow mesenchymal stromal/stem cells have demonstrated that the CCRP is present and can be regulated within osteoblast progenitors. In vivo studies have shown that the CCRP regulates bone mass via leptin/neuroendocrine pathways. The current study used an ovariectomized murine model to test the hypothesis that ovarian hormone deficiency is associated with either an attenuation and/or temporal phase shift of the CCRP oscillatory expression in bone and that these changes are correlated with the onset of osteoporosis. METHODS: Sham-operated controls and ovariectomized female C57BL/6 mice were euthanized at 4-h intervals 2 weeks post-operatively. RESULTS: Ovariectomy attenuated the oscillatory expression of CCRP mRNAs in the femur and vertebra relative to the controls and reduced the wheel-running activity profile. CONCLUSION: Ovarian hormone deficiency modulates the expression profile of the CCRP with potential impact on bone marrow mesenchymal stem cell lineage commitment.},
	keywords = {Animal Estrogens/deficiency/*physiology Female Femur/metabolism Gene Expression Profiling/methods Gene Expression Regulation/physiology Lumbar Vertebrae/metabolism Mice Mice, Animals Circadian Rhythm/genetics/*physiology Circadian Rhythm Signaling Peptides and Proteins/*biosynthesis/genetics Disease Models, Inbred C57BL Motor Activity/physiology Osteoporosis/genetics/metabolism/*physiopathology Ovariectomy RNA, Mechanical X-Ray Microtomography/methods, Messenger/genetics Stress},
	issn = {1433-2965 (Electronic) 0937-941X (Linking)},
	doi = {10.1007/s00198-010-1325-z},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/20593165},
	author = {Smith, B. J. and Sutton, G. M. and Wu, X. and Yu, G. and Goh, B. C. and Hebert, T. and Pelled, G. and Gazit, Z. and Gazit, D. and Butler, A. A. and Gimble, J. M.}
}
