@article {17103,
	title = {Micro-electroporation of mesenchymal stem cells with alternating electrical current pulses},
	journal = {Biomed Microdevices},
	volume = {11},
	number = {1},
	year = {2009},
	note = {Ziv, Roee Steinhardt, Yair Pelled, Gadi Gazit, Dan Rubinsky, Boris eng Research Support, Non-U.S. Gov{\textquoteright}t 2008/09/26 09:00 Biomed Microdevices. 2009 Feb;11(1):95-101. doi: 10.1007/s10544-008-9213-4.},
	pages = {95-101},
	type = {Journal Article},
	abstract = {Micro-electroporation is an electroporation technology in which the electrical field that induces cell membrane poration is focused onto a single cell contained in a micro-electromechanical structure. Micro-electroporation has many unique attributes including that it facilitates real time control over the process of electroporation at the single cell level. Flow-through micro-electroporation expands on this principle and was developed to facilitate electroporation of a large numbers of cells with control over the electroporation of every single cell. However, our studies show that when electroporation employs conventional direct current (DC) electrical pulses the micro-electroporation system fails, because of electrolysis induced gas bubble formation. We report in this study that when certain alternating currents (AC) electrical pulses are used for micro-electroporation it becomes possible to avoid electrolytic gas bubble formation in a micro-electroporation flow-through system. The effect of AC micro-electroporation on electrolysis was found to depend on the AC frequency used. This concept was tested with mesenchymal stem cells and preliminary results show successful electroporation using this system.},
	keywords = {Animals Cell Line Electroporation/*instrumentation/*methods Mesenchymal Stromal Cells/*cytology Mice Microfluidic Analytical Techniques/*instrumentation/*methods},
	issn = {1572-8781 (Electronic) 1387-2176 (Linking)},
	doi = {10.1007/s10544-008-9213-4},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/18815886},
	author = {Ziv, R. and Steinhardt, Y. and Pelled, G. and Gazit, D. and Rubinsky, B.}
}
