@article {17057,
	title = {Exogenously regulated stem cell-mediated gene therapy for bone regeneration},
	journal = {Mol Ther},
	volume = {3},
	number = {4},
	year = {2001},
	note = {Moutsatsos, I K Turgeman, G Zhou, S Kurkalli, B G Pelled, G Tzur, L Kelley, P Stumm, N Mi, S Muller, R Zilberman, Y Gazit, D eng Research Support, Non-U.S. Gov{\textquoteright}t 2001/04/26 10:00 Mol Ther. 2001 Apr;3(4):449-61.},
	pages = {449-61},
	type = {Journal Article},
	abstract = {Regulated expression of transgene production and function is of great importance for gene therapy. Such regulation can potentially be used to monitor and control complex biological processes. We report here a regulated stem cell-based system for controlling bone regeneration, utilizing genetically engineered mesenchymal stem cells (MSCs) harboring a tetracycline-regulated expression vector encoding the osteogenic growth factor human BMP-2. We show that doxycycline (a tetracycline analogue) is able to control hBMP-2 expression and thus control MSC osteogenic differentiation both in vitro and in vivo. Following in vivo transplantation of genetically engineered MSCs, doxycycline administration controlled both bone formation and bone regeneration. Moreover, our findings showed increased angiogenesis accompanied by bone formation whenever genetically engineered MSCs were induced to express hBMP-2 in vivo. Thus, our results demonstrate that regulated gene expression in mesenchymal stem cells can be used as a means to control bone healing.},
	keywords = {Animals Anti-Bacterial Agents/pharmacology Bone Development/genetics Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins/genetics Bone Regeneration/*genetics Cell Differentiation Cell Line Chick Embryo Doxycycline/pharmacology Female Fracture Healing, Fluorescence Neovascularization, Inbred BALB C Mice, Inbred C3H Microscopy, Physiologic Recombinant Proteins/genetics Reverse Transcriptase Polymerase Chain Reaction Stem Cells/*metabolism *Transforming Growth Factor beta Transgenes},
	issn = {1525-0016 (Print) 1525-0016 (Linking)},
	doi = {10.1006/mthe.2001.0291},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/11319905},
	author = {Moutsatsos, I. K. and Turgeman, G. and Zhou, S. and Kurkalli, B. G. and Pelled, G. and Tzur, L. and Kelley, P. and Stumm, N. and Mi, S. and Muller, R. and Zilberman, Y. and Gazit, D.}
}
